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KMID : 0861020130280010023
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Korea Journal of Herbology
2013 Volume.28 No. 1 p.23 ~ p.31
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Anti-obesity effects of herbal extract YY312 in C57BL/6 mice fed a high-fat diet and 3T3-L1 cells
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Kang In-Sug
Hwang Keun-Young
Choi A-Young
Roh Kug-Hwan
Choi Ji-Hyun
Sim Yeo-Moon
Park Yoo-Kyoung
Oh Myung-Sook
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Abstract
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Objectives : The purpose of this study was to determine the anti-obesity effect and molecular mechanism of
YY312, a herbal extract composed of Imperatae Rhizoma, Citri Unshius Pericarpium Immaturus, and Evodiae
Fructus, on a high-fat diet-induced animal model and on 3T3-L1 cells.
Methods : C57BL/6 mice were fed for 6 weeks with a normal diet or a high-fat diet (HFD). Then they orally
administered daily with 300 mg/kg YY312 for next 10 weeks. Body weight and food consumption were recorded
weekly and daily, respectively. Tissue weights, serum lipid, and glucose levels were analyzed at the end of the
study. Additionally, the effects of YY312 on adipocyte differentiation in 3T3-L1 cells were examined. After
differentiating 3T3-L1 cells were treated with YY312, Oil-red O staining, RT-PCR, and Western blotting were
performed for lipid accumulation, mRNA expression of adipogenesis gene, and AMP-activated protein kinase
(AMPK) phosphorylation, respectively.
Results : YY312-administered mice showed a significant reduction of body weights and abdominal adipose tissue
weights. YY312 also reduced the serum levels of triglycerides and total cholesterol, compared with the HFD group.
Treatment with YY312 inhibited lipid accumulation and blocked expression of adipogenic transcription factors and
lipogenesis genes, including peroxisome proliferator-activated receptor γ, CCAT/enhancer binding protein α and
fatty acid synthase. YY312 increased AMPK phosphorylation in 3T3-L1 adipocytes.
Conclusions : This study showed that herbal extract YY312 has an anti-obesity effect in vitro and in vivo.
Thus, YY312 could be developed as a supplement for reduction of body weight gain induced by an HFD.
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KEYWORD
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YY312, high-fat diet, obesity, C57/BL6 mice, body weight, 3T3-L1 adipocyte differentiation
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